Cracks in consolidants containing TiO2 as a habitat for biological colonization: A case of quaternary bioreceptivity.

The recently proposed concept of quaternary bioreceptivity applies to substrates treated with coating materials and it is considered in the present study with the alga Bracteacoccus minor and the cyanobacterium Nostoc sp. onto granite specimens treated with ethyl silicate and nano-sized silica doped with different amounts of TiO2 (0, 0.5, 1 and 3 wt%). The findings showed a lack of correlation between the amount of TiO2 and the level of colonization (main bioreceptivity estimator) to the presence of cracks on the surface, which annul the biocidal power of TiO2. Crack formation, which depends on the mechanical properties, greatly influences the bioreceptivity of the material. Thus, the cracks provided anchor points where water is retained, in turn strongly influencing the early stages of colonization kinetics, to a greater extent than the biocidal power of TiO2, which will probably increase as the biofilm develops over the entire surface. In addition, although the cracks were more abundant and wider in the ethyl silicate-based consolidant, the nano-sized silica provided better anchoring points, making the material treated with the corresponding consolidant more bioreceptive.

Revisiting and reanalysing the concept of bioreceptivity 25 years on.

2020 marks 25 years since Olivier Guillitte defined the term 'bioreceptivity', to describe the ability of a building material to be colonised by living organisms. Although Guillitte noted in his 1995 paper that several issues required further investigation, to the best of our knowledge the bioreceptivity concept has not been restated, reviewed, reanalysed or updated since then. The present paper provides an opinionated exposition of the status and utility of the bioreceptivity concept for built heritage science and conservation in the light of current knowledge, aimed to stimulate further discussion. A bibliometric analysis highlights the key dimensions of the past 25 years of published research, showing that the term bioreceptivity has been widely used in the field of built cultural heritage. In our reanalysis of the concept, special attention is devoted to the six types of bioreceptivity (primary, secondary, tertiary, intrinsic, extrinsic and semi-extrinsic) articulated by Guillitte in 1995. We propose that field-based studies of bioreceptivity are urgently needed, and that the intrinsic, extrinsic and semi-extrinsic types of bioreceptivity should be dropped, and a new category (quaternary bioreceptivity) added. Additionally, we propose that bioreceptivity in submerged and subsoil environments should also be considered. Bioreceptivity remains an important concept for managing both new build and built heritage, as it provides the key to understanding the drivers and patterns of biological colonisation of building materials.

Impact of wildfire on granite outcrops in archaeological sites surrounded by different types of vegetation.

The lack of scientific information about the effects of wildfire on prehistoric structures and rock art, such as dolmens and petroglyphs, impedes the development of conservation guidelines. In this study, the impact of a recent wildfire (late 2017) on granite outcrops in the San Salvador de Coruxo archaeological site (Vigo, SW Galicia) was evaluated. Samples of the same type of granite were obtained from three sites characterised by different types of vegetation (natural scrub, native deciduous oak and non-native pine-eucalypt forest) in order to determine how the vegetation influences the fire-caused damage to the rock. Three subsamples were taken from each of the granite samples at depths of 1 cm-3 cm to study how fire affects the rock at depth. In all sites, the temperature reached at the granite surface was below 380 °C. No mineralogical changes due to fire exposure were detected, and no physical changes that could be attributed to the effect of the fire on the fissure system of the granite were identified. However, aesthetic colour changes due to the deposition of organic and charred matter, which even penetrated the fissures, were detected. The existence of lignin-derived compounds, lipids and carbohydrates in the samples from the oakwood site indicates greater resistance to fire effects in this type of vegetation than in the other two types. Although preliminary, these findings suggest that oakwoods could act as protective belts around archaeological sites by reducing the wildfire severity, because of their greater resistance to being burnt, and that they could buffer the damaging effects of fire in natural areas where parietal art is found.

Ensayos clínicos, ciencia y economía / Clinical trials, science and economy

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Bioreceptivity of building stones: a review.

In 1995, Guillitte defined bioreceptivity, a new term in ecology, as the ability of a material to be colonized by living organisms. Information about the bioreceptivity of stone is of great importance since it will help us to understand the material properties which influence the development of biological colonization in the built environment, and will also provide useful information as regards selecting stones for the conservation of heritage monuments and construction of new buildings. Studies of the bioreceptivity of stone materials are reviewed here with the aim of providing a clear set of conclusions on the topic. Definitions of bioreceptivity are given, stone bioreceptivity experiments are described, and finally the stone properties related to bioreceptivity are discussed. We suggest that a standardized laboratory protocol for evaluating stone bioreceptivity and definition of a stone bioreceptivity index are required to enable creation of a database on the primary bioreceptivity of stone materials.

Spectrophotometric color measurement for early detection and monitoring of greening on granite buildings.

This paper addresses the detection and monitoring of the development of epilithic phototrophic biofilms on the granite façade of an institutional building in Santiago de Compostela (NW Spain), and reports a case study of preventive conservation. The results provide a basis for establishing criteria for the early detection of phototrophic colonization (greening) and for monitoring its development on granite buildings by the use of color changes recorded with a portable spectrophotometer and represented in the CIELAB color space. The results show that parameter b* (associated with changes of yellowness-blueness) provides the earliest indication of colonization and varies most over time, so that it is most important in determining the total color change. The limit of perception of the greening on a granite surface was also established in a psycho-physical experiment, as Δb*: +0.59 CIELAB units that correspond, in the present study, to 6.3 µg of biomass dry weight cm(-2) and (8.43 ± 0.24) × 10(-3) µg of extracted chlorophyll a cm(-2).

Degradation of nitrocellulose-based paint by Desulfovibrio desulfuricans ATCC 13541.

Nitrocellulose is one of the most commonly used compounds in ammunition and paint industries and its recalcitrance to degradation has a negative impact on human health and the environment. In this study the capability of Desulfovibrio desulfuricans ATCC 13541 to degrade nitrocellulose as binder in paint was assayed for the first time. Nitrocellulose-based paint degradation was followed by monitoring the variation in nitrate, nitrite and ammonium content in the culture medium using Ultraviolet-Visible spectroscopy. At the same time cell counts and ATP assay were performed to estimate bacterial density and activity in all samples. Infrared spectroscopy and colorimetric measurements of paint samples were performed to assess chemical and colour changes due to the microbial action. Microscope observations of nitrocellulose-based paint samples demonstrated the capability of the bacterium to adhere to the paint surface and change the paint adhesive characteristics. Finally, preliminary studies of nitrocellulose degradation pathway were conducted by assaying nitrate- and nitrite reductases activity in D. desulfuricans grown in presence or in absence of paint. We found that D. desulfuricans ATCC 13541 is able to transform nitrocellulose as paint binder and we hypothesised ammonification as degradation pathway. The results suggest that D. desulfuricans ATCC 13541 is a good candidate as a nitrocellulose-degrading bacterium.

Color measurements as a reliable method for estimating chlorophyll degradation to phaeopigments.

The application of biocides is a traditional method of controlling biodecay of outdoor cultural heritage. Chlorophyll degradation to phaeopigments is used to test the biocidal efficacy of the antimicrobial agents. In the present study, the usefulness of color measurements in estimating chlorophyll degradation was investigated. An aeroterrestrial stone biofilm-forming cyanobacterium of the genus Nostoc was chosen as test organism, comparing its different behaviour in both planktonic and biofilm mode of growth against the isothiazoline biocide Biotin T®. Changes in A(435 nm)/A(415 nm) and A(665 nm)/A(665a nm) and in the chlorophyll a and adenosine triphosphate (ATP) cell content were compared with the variations in the CIELAB color parameters (L*, a*, b*, C*(ab) and h(ab)). Our findings showed that both the phaeophytination indexes are useful in describing degradation of chlorophyl a to phaeopigments. Moreover, the CIELAB color parameters represented an effective tool in describing chlorophyll degradation. L* CIELAB parameter appeared to be the most informative parameter in describing the biocidal activity of Biotin T® against Nostoc sp. in both planktonic and biofilm mode of growth.

Quantification of phototrophic biomass on rocks: optimization of chlorophyll-a extraction by response surface methodology.

Biological colonization of rock surfaces constitutes an important problem for maintenance of buildings and monuments. In this work, we aim to establish an efficient extraction protocol for chlorophyll-a specific for rock materials, as this is one of the most commonly used biomarkers for quantifying phototrophic biomass. For this purpose, rock samples were cut into blocks, and three different mechanical treatments were tested, prior to extraction in dimethyl sulfoxide (DMSO). To evaluate the influence of the experimental factors (1) extractant-to-sample ratio, (2) temperature, and (3) time of incubation, on chlorophyll-a recovery (response variable), incomplete factorial designs of experiments were followed. Temperature of incubation was the most relevant variable for chlorophyll-a extraction. The experimental data obtained were analyzed following a response surface methodology, which allowed the development of empirical models describing the interrelationship between the considered response and experimental variables. The optimal extraction conditions for chlorophyll-a were estimated, and the expected yields were calculated. Based on these results, we propose a method involving application of ultrasound directly to intact sample, followed by incubation in 0.43 ml DMSO/cm(2) sample at 63°C for 40 min. Confirmation experiments were performed at the predicted optimal conditions, allowing chlorophyll-a recovery of 84.4 ± 11.6% (90% was expected), which implies a substantial improvement with respect to the expected recovery using previous methods (68%). This method will enable detection of small amounts of photosynthetic microorganisms and quantification of the extent of biocolonization of stone surfaces.

Relationship between color and pigment production in two stone biofilm-forming cyanobacteria (Nostoc sp. PCC 9104 and Nostoc sp. PCC 9025).

Previous studies have provided evidence that color measurements enable on site quantification of superficial biofilms, thereby avoiding the need for sampling. In the present study, the efficiency of color measurements to evaluate to what extent pigment production is affected by environmental parameters such as light intensity, combined nitrogen and nutrient availability, was tested with two cyanobacteria, Nostoc sp. strains PCC 9104 and PCC 9025, which form biofilms on stone. Both strains were acclimated, in aerated batch cultures for 2 weeks, to three different culture media: BG-11, BG-11(0), and BG-11(0)/10 at either high or low light intensity. The content of chlorophyll a, carotenoids, and phycocyanins was measured throughout the experiment, together with variations in the color of the cyanobacteria, which were represented in the CIELAB color space. The results confirmed that the CIELAB color parameters are correlated with pigment content in such a way that variations in the latter are reflected as variations in color.

[Quality control of total parenteral nutrition in the 1991-1992 biennium]. / Control de calidad de la nutrición parenteral total en el bienio 1991-1992.

OBJECTIVE: To assess the degree of compliance with standards defined for Total Parenteral Nutrition (TPN) quality control in our hospital in the two-year period 1991-1992. MATERIAL AND METHODS: All available information was assessed concerning 52 patients (32 men and 17 women) for whom a TPN course was prescribed in relation with certain pre-set indications. These patients received a total of 1140 TPN units with the TPN lasting an average of 21.9 days (range, 1-73 days). Monitoring was done by the Nutrition and Diet Section. The degree of compliance was assessed with the standards model proposed by the Providence Medical Center, Portland (USA) (PMCP) with 24 parameters (PM): indications, initiation in the first 24 hours, nutritional assessment in the first 24 hours, period of the TPN, metabolic complications (14 PM), septic complications (3 PM), nutritional consequences (2 PM) and TPN losses. All parameters were appraised and admitted, except for urea, which was corrected to standard values of our Laboratory (< 44 mg/dl), with Transferrin evaluation instead of iron binding capacity (TIBC), taking as compliance standard a figure of > 190 mg/dl. All analytical calculations were carried out in our Central Laboratory Service. RESULTS: Of all the parameters, the following were discarded, not being calculated on a routine basis: total CO2, serous magnesium, urinary uric nitrogen, nitrogen balance, positive hemocultures, catheters and TPN losses. Levels of compliance varied between 31.9% and 100%, with 8 parameters within the standards (Initiation, 100%; evaluation in first 24 hours, 100%; extent, 100%; creatinine, 100%; total bilirubin, 92.2%; cholesterol, 99.5%; transferrin, 35.9%). Seven parameters fell short (Na, 88.1%; K, 92.9%; Cl, 89.3%; Urea, 54.4%; Glucose, 96.4%; P, 94.1%; Triglycerides, 71.9%). CONCLUSIONS: We infer from our study that there is a need to make use of a large part of the indicators described in the literature as indicators for quality guarantee of a TPN program, and the use of new parameters must be assessed in normal monitoring.